Course supervisor: Trond Stokke Trond.Stokke@rr-research.no
Venue: Radiumhospitalet, Montebello, Ullernchausséen 70. See the program below for details
Program: Flow cytometry course (MF 9170) 11–13/4 2018
Wednesday 11/4 (Montebello, meetingroom 4.th floor K-building):
0900-1030 Principles for FCM-Fluorescence/light scatter (Stokke)
Break
1045-1130 More about flow cytometers (Stokke)
1130-1215 Histograms, cytograms and data treatment (Stokke)
1215-1300 Lunch
1300-1345 Sorting (Dale Rein)
Kaffepause
1400-1600 FCM software/DiVa/Modfit (Stokke)
1400-1600 Demo flow cytometer: FacsVantage and LSRII (Bostad/Notø)
Thursday 12/4 (NB! In courseroom J4051, 4.th floor in the Radiation building):
0900-1015 Immunofluorescence I (Stokke)
Break
1030-1100 The cell cycle/analysis of DNA-histograms/ploidy/BrdU (Stokke)
1100-1215 Lab (DNA-histogram-analysis, Stokke, Bostad)
1215-1300 Lunch
1300-1330 Miscellaneous applications for FCM/ DNA staining (Stokke)
1330-1400 New instrumentation – CyTOF (Stokke)
Break
1415-1600 Lab (Leukocyte classification/analysis, Stokke, Bostad)
Friday 13/4 (Montebello, meetingroom 4.th floor K-building):
0900- 0945 Immunofluorescence II (Stokke)
Break
1000-1045 Multiparameter flow cytometry and compensation (Rasmussen)
1045-1145 Signal transduction by flow cytometry (Myklebust)
1145- 1245 Lunch
1245-1345 Apoptosis (Dale Rein)
1345 - 1430 Presentation of data (Landsverk)
1430-1600 Written exam (Stokke)